Intermediate

Part:BBa_K569000:Design

Designed by: Mitu Bhattatiry   Group: iGEM11_UT_Dallas   (2011-09-27)


RBS+LuxI+dblterm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to design the primers to have the correct BioBrick enzymes so that we could ligate in new promoters.


Source

We used primers CCA.TCTAGA.AGCACTACTAGAGAAAGAGGAGAAATA, and GCCTTTGAGTGAGCTGATACC to PCR out the part from BBa_J23039 from Berkeley 2006.

References